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KMID : 1134820180470060664
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Journal of the Korean Society of Food Science and Nutrition
2018 Volume.47 No. 6 p.664 ~ p.668
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Protective Effects of Ethanol Extracts from Selected Food Materials against UVB-Induced Damage in Human Skin Fibroblast (Hs68) Cells
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Lee Ha-Na
Lee Jong-Min
Hong Sung-Moon
Kim Na-Young
Son Seok-Jun
Jeong Young-Jae
Lee Hyun-Soon
Lee Jun-Soo
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Abstract
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Ultraviolet B (UVB) irradiation is a major cause of photoaging and leads to the formation of skin wrinkles by degrading collagen via activation of matrix metalloproteinases (MMPs). In this study, we investigated the protective effects of ethanol extracts from selected food materials against UVB-induced photoaging in human skin fibroblast (Hs68) cells. The protective effects of the samples on cell viability were examined by MTT assay under UVB (30 mJ/cm2)-treated and untreated conditions. Intracellular levels of reactive oxygen species (ROS) were determined by using a fluorescent spectrophotometer. The levels of MMPs and collagen were measured by assay kits. The extracts (50 μg/mL) did not show any cytotoxicity. Extracts from red paprika, tomato, pumpkin, chestnut shell, and Jinuni bean showed protective effect against UVB irradiation (88, 104, 98, 97, and 88%, respectively). These extracts reversed elevation of ROS production and prevented reduction of collagen levels. Moreover, the extracts inhibited elevation of MMP-1 and MMP-3 levels caused by exposure to UVB irradiation in Hs68 cells. Among the samples tested, chestnut shell was found to exhibit the strongest inhibitory effect against elevation of UVB-induced MMPs. Taken together, these data suggest that chestnut shell is the most promising food material for treatment of UVB-induced photoaging in cosmetic products.
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KEYWORD
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photoaging, fibroblast, collagen, matrix metalloproteinase, foods
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